HPLC Troubleshooting - Split Peaks

If your chromatography has recently demonstrated this phenomena of split peaks some common causes are described below.

Causes	Solutions
Soiled guard or column inlet	- Replace guard or inline filter frit; reverse flush column (if permitted).
Sample diluent incompatible with mobile phase	- Change sample diluent. Use initial mobile phase solvent composition (if applicable). Use Co-Solvent or POISe injection function.
Analyte properties	- Possibility of isomer or analyte interconversion – alter conditions to correct for this.