If your chromatography has recently been losing resolution; common causes are described below.
| Causes | Solutions |
| Changes in peak width | - Changes in column performance and sample load / column efficiency can result in wider peak widths. Ensure the chromatographic performance of the column is sufficient, or replace the column, and ensure the same load is consistent. |
| Changes in retention time | - See changes in retention time section. |
| Mobile phase deterioration or evaporation | - Prepare fresh mobile phases. |
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