If you noticed baseline drift with your chromatography; common causes are listed below.
| Causes | Solutions |
| Column temperature fluctuation | - Control column temperature. |
| Low quality mobile phase | - Use HPLC gradient grade solvents, high purity salts and additives. Ensure mobile phases are degassed sufficiently. |
| Contaminant or air bubble in detector flow cell | - Flush flow cell with isopropanol. If necessary, clean cell with 1N nitric acid. |
| Cracked cell window | - Replace flow cell window. |
| Mobile phase mixing issue | - Use larger or more efficient mixer. |
| Slow column equilibration | - Flush column with at least 10-20 column volumes with new mobile phase. |
| Strongly retained materials with high capacity factor eluting in subsequent injections | - Use a strong flush procedure between injections or if permitted backflush column with strong solvent between injections for more challenging strongly retained contaminants. |
| Mobile phase recycled but, detector not auto-zeroed correctly | - Auto-zero detector. |
| Detector (UV) not set at absorbance maxima but, on slope of curve | - Change wavelength to UV absorbance maxima. |
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