If you observe a change in chromatography with a noisy baseline; some common causes are listed below.
| Causes | Solutions |
| Air in detector cell or pump | - Degas mobile phase sufficiently and purge lines and flow path. |
| Leak | - Check wettable flow path and fittings. |
| Incomplete mobile phase mixing | - Pre-mix mobile phase or use larger mixer. |
| Temperature variance at detector | - Use temperature-controlled detector flow cell. |
| Mobile phase contaminated or deteriorated | - Check mobile phase. |
| Mobile phase solvents immiscible (Pressure spikes can also be observed) | - Use miscible mobile phases. |
| Air trapped in system | - Flush and purge flow path. |
| Weak detector lamp | - Replace lamp. |
| Column leaking silica or packing material | - Replace column. |
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