HPLC Troubleshooting - Low Pressure

If your LC system is demonstrating lower than expected system pressure; the following causes may be responsible.

Causes	Solutions
Partial leak in system	- Check all connections and retighten any which have leaks.
Flow rate	- Check the method has the correct flow rate. - Test the flow rate accuracy using a calibrated flow rate meter or collect a specific volume and monitor the time required.
Method	- Check if method is using correct temperature and correct solvents.
Incorrect column	- Use correct column with correct dimensions and particle geometry.
Column temperature too high	- Set adequate column temperature and check no column damage if exceeded column temperature limit.
Sensor malfunction	- Repair or replace pressure sensor.