When is Gradient Chromatography Used?

When is Gradient Chromatography Used?

The use of isocratic or gradient conditions are completely application driven, thus care should be given to select the most appropriate conditions. Some examples where gradient analysis is applied include:

• To elute a range of polar to non-polar compounds within a reasonable timeframe.
• To clean the column in preparation for the next run. This is particularly important for samples with matrices to ensure all contaminants are removed from the stationary phase.
• To find the optimum method conditions using gradient scouting runs to build a retention model. The retention model can enable the most robust and suitable resolution to be achieved for a given separation.
• For peptide, protein and oligo analysis, which are highly susceptible to small changes in organic.