Generic Reversed Phase Column Storage

Generic Reversed Phase Column Storage

A proportion of organic is required to prevent microbial growth, however, 100% organic can cause leeching of metals from the steel column, which can have adverse effects on chromatographic performance. Acetonitrile is often preferable to methanol as certain stationary phase ligands are not stable for long term storage in methanol. Methanol is slightly acidic therefore if a ligand is susceptible to hydrolysis the stationary phase can be cleaved.

IMPORTANT: There are exceptions to this, such as ion exchange stationary phases, which have specific storage conditions. It is therefore good practice to check the advice from the manufacturer on column storage conditions.