Purification Tips: Volume Load versus Concentration Load with SFC

Injection volume and sample concentration are studied to select the proper loading amount. There are two methods to increase loading amount: “Concentration Load” (increasing concentration of the sample with fixed injection volume) and “Volume Load” (increasing injection volume with fixed concentration of the sample).

Loading condition studies are recommended to be conducted with Concentration Load because the peak shape is less influenced by Concentration Load than by Volume Load. Volume Load can be used in combination of Concentration Load.

Peak shapes can be affected by sample solvents. It is preferable for samples to be dissolved in mobile phases or solutions with lower eluting power.