Rob Stimpson

1. Pull up the guide (1) and lift up the lower chamber (3).2. Put the upper chamber (2) onto the lower chamber together with the spacer (4) which is for the underside.     3. Pull down the guide, ...

1. Remove the inlet tube fixing screw.     2. Loosen the outlet tube fixing screw.  NOTE: To Loosen a little is enough. Do not remove it.     3. Remove the reactor fixing screw.     4. Lift up the ...

When not using the system for a long time, in consideration of the next startup, perform the following procedures before turning OFF the power.   CAUTION: Clean the column of the gradient system ...

When not using the system for a short time, replace the liquid in the column and piping with gradient solution B and then turn off the power to the PPSQ, detector, and column oven. (However, in ord...

When not using the system for a long time, in consideration of the next startup, perform the following procedures before turning OFF the power. 1. Cleaning the Flow Line  1) Set a new glass fiber d...

When not using the system for a short time, it is sufficient to leave the pump unit pumping at a flow rate of 0.2 to 0.3mL/min and to turn OFF the power to the PPSQ, the detector, and the column ov...

The isocratic system allows pyridylethylation of cysteine in a PPSQ reactor preceding sequence analysis.The sequence schedule for pyridylethylation is installed in the PPSQ.   NOTE: Pyridylethylati...

If a sample with a high salt content is analyzed with the protein sequencer, the background level in the chromatograms obtained may be high or the mobile phase may be contaminated. In this case, th...

The sequence analysis of a peptide solution sample is usually performed using a polybrene-processed glass fiber disk as the sample support. With a PVDF membrane, because the holding power for pepti...

« Analysis takes longer to complete » • K is close to I and separation is poor. →The column temperature is too low.: Check the column temperature.(40ºC)   • In general, the retention of amino acid...